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BBT055 BBT055 Bioanalytical chemistry lp3 VT23 (7.5 hp)

Course is offered by the department of Biology and Biological Engineering

 

Course Plan

Examiner: Dr. Otto Savolainen (OS), e-mail otto.savolainen@chalmers.se

 

Course goal

This introductory course is intended to provide a fundamental understanding of the theoretical and practical aspects of analysis of biomolecules and bio-relevant molecules, including various modern instrumental techniques, sampling protocols, sample pre-treatment, and data processing used in modern bioanalysis. It will help students to think critically and make informed choices between proposed analytical techniques, methods and instruments in order to solve a bioanalytical problem.

A major goal of the course is to enable students to select analytical approaches for bioanalysis, and interpret data. The course will teach the principles of quality control and assurance and enable students to apply their knowledge to the practical solution of real analytical problems. The course will also provide an insight into current analytical literature to enable students to review, select, read and critically discuss resources most relevant to a particular topic. The students will further develop practical skills in laboratory experiments, and the techniques of communication, including the writing of laboratory reports.

 

Course Textbooks

1.   Quantitative Chemical Analysis Daniel Harris, 6th. Ed. or later. An alternative e-book: Analytical Chemistry 2.1 - D. Harvey (free download, No MS covered in this free e-book) http://dpuadweb.depauw.edu/harvey_web/eTextProject/version_2.1.html

  1. S.R. Mikkelsen & E. corton: Bioanalytical Chemistry 2nd. Ed or later.

 

Recommended Reading Chapters in the Quantitative Analytical Chemistry textbooks

Subject

Book Chapter

Ebook chapter

The analytical approach, sample preparation

28

7

Separations

23

12

Statistics, data analysis

4

n.a.

Chromatography Theory

26

12B

Gas Chromatography

24

12D

Liquid Chromatography

25

12E

Spectrophotometry

19, 20

10B, 10C

Atomic and Molecule Spectroscopy

21

10D, 10G

Mass Spectrometry

22

n.a.

Electroanalytical Chemistry

14, 15, 17

11

Capillary Electrophoresis, Ion Chromatography

26

12F, 12G

Applications, method selection

25-3, 25-4

14

                                                                     

 Recommended Reading Chapters in the Bioanalytical Chemistry textbook

Subject

Book Chapter

Analysis of specific biomolecules

3,4,5- enzymes, 6- antibodies

Spectroscopy

2

Immunoassays

7

Biosensors

8

Electromigration

11-14

Imaging

10

Chromatography

16

Mass spectrometry

17

Chip technologies

18

 

Course Structure

The course contains lectures, 3 laboratory exercises and seminars/journal club. Please see the schedule in Timeedit and in Canvas.

 

Course requirements

  • Tentamen: According to Tentamen schedule.
  • 3 half day laboratory experiments and submission of all lab reports within one week after the experiment. One report per group is sufficient, but individual reports are also accepted.
  • Seminars/Journal club: Active participation to all Journal Club work.

 

Laboratory Experiments and Laboratory Seminars:

The course includes 3 half day laboratory exercises concentrating on fundamental work in Spectroscopy, HPLC, and GC-MS. Each exercise includes writing of a report. The groups meet in Kurs Huset Lab 5117 on the designated days/times. Assigning to lab groups is done in Canvas. Maximum number of students per group is preliminary 6. The laboratory exercises takes approximately 4 h according to the timetable available in Canvas.

 

A compulsory introductory seminar into the laboratory work is held to discuss the experiments and their background, and safety regulations (see the timetable in Canvas). Topics discussed in the lab seminarsinclude background and aim of the experiment, experimental method and setup, principle of the analysis, steps to be performed in the experiment.

 

Note! You are required to complete a risk assessment and read and understand the lab manuals and the underlying theory before your group starts with the practical experiments. Contact the lab assistant or the lecturer if you have general troubles with the risk assessment or the manual.

 

Laboratory Supervisors:

Laboratory supervisors can decide for exceptions from the lab report guidelines.

 

Journal club

The course includes a Journal Club exercise that includes reading and analyzing scientific articles relevant for the course content, as well as presenting an article in groups and acting as an opponent. Written instructions and schedule for Journal Club are provided on Canvas.  

 

Contact for students with special needs:

Annelie Karlsson. Email: funka@chalmers.se

phone: +46 (0)31-772 25 28

 

Course documents

All course documents are made available on Canvas.

 

Course summary:

Date Details Due