Course syllabus
Course-PM
KBT315 KBT315 Advanced analytical chemistry lp2 HT25 (7.5 hp)
Course is offered by the department of Chemistry and Chemical Engineering
Contact details
Examiner: Ann-Sofie Cans - E-mail cans@chalmers.se
Teachers:
Jan-Olof Svensson (JOS), Astra Zeneca. JanOlof.Svensson@astrazeneca.com
Per Malmberg (PM), Chalmers malmper@chalmers.se
Carl Brunius (CB), Chalmers carl.brunius@chalmers.se
Magnus Fransson (MF) Astra Zeneca Magnus.Fransson@astrazeneca.com
Ann-Sofie Cans (ASC), Chalmers cans@chalmers.se
Laboratory supervisors:
Caroline Lööf caroline.loof@chalmers.se
Rui Liu rui.liu@chalmers.se
Daniel Hoffmann danhof@chalmers.se
Course purpose
This advanced analytical chemistry course is intended to provide a deepened understanding of the practical aspects of chemical analysis, including design of sampling protocols, sample pre-treatment, data handling and of the instrumentation used in modern chemical analysis. The students will practice thinking critically and make informed choices between proposed analytical instrumentations and methods.
The course will teach the principles of quality control and assurance and enable students to apply their knowledge to the practical solution of real analytical problems. The course will provide familiarity with current analytical literature to enable you to review, select, read, and critically discuss the papers most relevant to a particular topic. Students will further develop the techniques of communication, including report writing and oral presentation, and will receive training in independent literature study..
Schedule
Course literature
Course Textbook: The course is based on the fundamental principles covered in Quantitative Chemical Analysis by Daniel Harris, 6th. Ed. or later but does not follow the book structure. Individual textbook study is highly recommended to be able to pass the in-class test and participate efficiently in the seminars.
Recommended alternative: Analytical Chemistry 2.1 - D. Harvey
This e-book is free of charge to download. However, this book offers no section on the topic mass spectrometry. The book can be downloaded here:
http://dpuadweb.depauw.edu/harvey_web/eTextProject/version_2.1.html
Reading Chapters in the course textbook by Harris and the eBook by Harvey:
|
Topic |
Chapter D.C. Harris |
Chapter D. Harvey |
|
The analytical process & sample preparation |
28 |
7, 18 |
|
Chemical separations |
23 |
12 |
|
Chromatography Theory |
26 |
12B |
|
Gas Chromatography |
24 |
12D |
|
Liquid Chromatography |
25 |
12E |
|
Spectroscopy methods |
19,20,21 |
10B, 10C, 10 D, 10 G |
|
Mass spectrometry |
22 |
|
|
Electroanalytical Chemistry |
14,15,17 |
11 |
|
Uncertainties in analytical measurements |
3 |
4 |
|
Figure of merits, calibrations, validation |
5 |
3 |
The chapters in the different editions of Harris vary, but the content of each chapter is fully sufficient no matter the edition. Material related to mass spectrometry and biosensors will be handed out in connection to the mass spectrometry lectures.
Course design
Lectures: The course is structured with lectures at Tuesdays 13.15-15.00 and 15.15 - 17.00, with one exception to also one Thursday afternoon on the 6th of November.
Laboratory work: Two problem-solving labs are included in the course, where student will use the available equipments LC-MS, GC-MS and UV/VIS. Laboratory experiments are scheduled for 10 half day exercises where the pre-assigned groups meet at the designated dates according to the lab schedule and lab groups in Kurshuset Lab 5117. Admission into the lab requires wearing a lab coat and proper shoes. Safety glasses are provided.
Students are handed material to read and process before showing up to the labs. This will also improve the speed of your experimental success. This course offers training in the analytical process, with focus to training in designing your own lab manuals, process data and present the experimental results. Guidelines are provided to the following two lab assignments:
- Determination of the concentration of gold nanoparticles in a sample solution
- Determination of the amount xylitol in chewing gum
Literature assignments presentations
The course includes a mandatory individual literature project where each student will write individual project report and present the project at a workshop at the end of this course.
On Tuesday 16th of December 13.15-17.00 and Thursday 18th of December 13.15-17.00 these individual literature projects will be presented.
Quiz: During the course, three online quiz will be published at Canvas to test your knowledge gained from lectures.
Guidelines for quiz:
The three tests contain basic questions and calculations. The test is evaluated to measure your ongoing performance during the course. The evaluation does not influence the final course grade. You have to reach at least 50% of the score in each test to pass the course. You will be given a total of 10 attempts to perform the quiz. If you score less than 50% in the test after the 10 attempts, you will have to prepare a home assignment on the failed subjects. Failure to produce this essay within two weeks will result in failure of the course.
Questions about the course can be e-mailed to the teachers and supervisors, where general questions can be directed to the examiner.
Changes made since the last occasion
The last year of 4 hour lecture in chromatography has now been divided up in a 2-hour recap lecture of basic chromatography aimed at students that did not take the basic course in analytical chemistry and students that wish a refresher on the topic. A 2 hour advanced chromatography lecture with will subsequently be given with material that is new to all students in this course.
A 2 hour recap lecture on basic analytical chemistry will also be offered to students that did not previously take the basic analytical course or for students that wish to have a refresher.
The lab on acetaminophen analysis using GC-MS or HPLC-UV/VIS have been replaced by a lab determining the amount xylitol in chewing gum using GC-MS. This new lab offers experience using sample prep techniques before chromatographic analysis of the sample.
An additional lecture on chemometrics following the statistics and dataanlysis have been added.
Learning objectives and syllabus
Learning objectives:
- explain the analytical process
- select appropriate analytical techniques and methods for a given problem including sample pre-treatment, instrumental techniques, method validation and data processing
- understand the use of multivariate statistics to a variety of data with emphasis on spectroscopic data
- explain and compare analytical techniques
- use GC-MS, LC-MS, UV/VIS
- design and modify analytical protocols
- interpret mass spectra obtained by GC-MS or LC-MS
- apply good laboratory practice
- evaluate and critically examine data and literature
- present results in relation to state of the art, both written and orally.
- understand the ethical problems and principles in science and engineering with specific focus on the analytical sciences
Link to the syllabus on Studieportalen.
Examination form
- Literature assignment:: Paper confirmation: Thursday 13th of November. One individual report to be handed in until Wednesday 10th of December
- Course workshop: Exam equivalent: presence is mandatory for everyone in the session where you present. Written guidelines are provided.
- Two different laboratory experiments and submission of a lab report for each of the two experiments. One report per group is sufficient, but individual reports are also accepted. Written guidelines are provided.
- Quiz tests: Three different quiz will be available on Canvas on the 12th of November, 3rd of December and 10th of December. The test is to assess your study progress based on lecture material topics. The in-class test is composed of questions regarding basic subjects complementing the lectures. Written guidelines are provided.
Course summary:
| Date | Details | Due |
|---|---|---|